A4418
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A4418
Sigma
Ammonium sulfate
for molecular biology, ≥99.0%
| CAS Number: | 7783-20-2 |
| Linear Formula: | (NH4)2SO4 |
| Molecular Weight: | 132.14 |
| EC Number: | 231-984-1 |
| MDL number: | MFCD00003391 |
| PubChem Substance ID: | 24890861 |
Description
| Application | May be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins. |
Properties
| grade | for molecular biology |
| assay | ≥99.0% |
| total impurities | ≤0.005% Insolubles |
| mp | >280 °C (dec.)(lit.) |
| density | 1.77 g/mL at 25 °C(lit.) |
| anion traces | chloride (Cl-): ≤5 ppm |
| cation traces | Cu: ≤5 ppm |
| Fe: ≤5 ppm | |
| Zn: ≤5 ppm | |
| heavy metals (as Pb): ≤2 ppm | |
| abs. | A |
| A | |
| foreign activity | DNase, RNase, and protease, none detected |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 1 |
| RTECS | BS4500000 |
References
| reference | Englard, S. and Seifter, S., Precipitation techniques. Meth. Enzymol. 182, 285-300, (1990) |
| Kent, U.M., Purification of antibodies using ammonium sulfate fractionation or gel filtration. Methods Mol. Biol., (1999) 115, 11-18 | |
| Dock, A.C., et al., Crystallization of transfer ribonucleic acids. Biochimie 66, 179-201, (1984) | |
| Yaremchuk, A., et al., Improved crystals of Thermus thermophilus prolyl-tRNA synthetase complexed with cognate tRNA obtained by crystallization from precipitate. Acta Crystallogr. D (Biol. Crystallogr.) 56, 197-199, (2000) | |
| Merck | Merck 13,559 |
| FT-IR 2 (3), 4751:A / FT-IR 1 (2), 1261:B / IR-Spectra (3), 1521:E / IR-Spectra (3), 762:C / RegBook 1 (3), 3439:D / Sax 6, 268 |
